High-performance liquid chromatographic analysis of bradykinin

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC DETERMINATION OF THEOPHYLLINE IN HUMAN SERUM

A simple, rapid and sensitive HPLC method for the determination of theophylline (T) in human serum has been developed. An isocratic system consisting of a /-l Bondapak C18 column, mobile phase of methanol, phosphate buffer (22:78, pH=4.5), and a flow rate of 1.4 mL/min was used. The eluent was detected by UV at 275 nm at room temperature. 8-Chlorotheophylline (8-CT) was used as an internal ...

HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC ANALYSIS OF SULFUR MUSTARD REACTION WITH AMINO ACIDS AND PROTEINS

The interaction of sulfur mustard with aminoacids and proteins has been investigated in this study. Rats were injected with sublethal doses of sulfur mustard subcutaneously and intraperitoneally. At different time intervals, plasma and urine samples were collected. The binding affinity of sulfur mustard with urinary and plasma proteins and enzymes was studied for the first time using non-i...

High performance liquid chromatographic analysis of sugars in raw and processed potatoes

High-performance liquid-chromatographic analysis for tryptophan in serum.

Concentrations of total tryptophan were determined rapidly and sensitively in 50 microliter of serum by a reversed-phase partition version of high-performance liquid chromatography. For determination of total tryptophan, sample preparation requires only precipitation of the serum protein with trichloroacetic acid and removing excess trichloroacetic acid with a 1,1,2-trichlorotrifluoroethane(Fre...

High performance liquid chromatographic analysis of reduction products of a thiolated DNA for immobilization on gold nanoparticles

DNA-based nano-biosensors are emerging scope in the field of biosensors. Many synthetic single stranded functional DNAs have been applied for development of such sensors, recently. Immobilization of DNA oligonucleotides on the surface of gold nanoparticles is a key step in the development of most colorimetric nano-biosensors. The bound DNA is usually thiolated and forms Au-S covalent bond to th...